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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Maize with altered growth and tolerance to herbicides
EN
pUBIL::GA20OX1
No
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Person:Prof. Dr. Frank Van Breusegem, Departement Planten SysteembiologieTechnologiepark 927Gent,
9052, BelgiumPhone: + 32 (0)9 33 13 920,Fax:Email: frbre@psb.vib-ugent.be,Website: http://www.vib.be,Related OrganizationVIB-UGent ()Academic or research instituteTechnologiepark 927Gent,
9052, BelgiumPhone: + 32 (0)9 33 13 920,Fax:Email: frbre@psb.vib-ugent.be,Website: http://www.vib.be,
The genetically modified maize has an altered growth characteristic resulting from the introduction of a Gibberelin 20 Oxidase-1 gene from Arabidopsis thaliana to increase levels of gibberellic acid. The plants become significantly longer and more slender without producing significantly more biomass. At the cellular level, the plants produced more cellulose, lignin, and cell wall residue.
Additionally the bar-gene from Streptomyces hygroscopicus is present as a selectable marker gene. The gene encodes phosphinotricin acetyl transferase enzyme (PAT), which acetylates phosphinotricin, also known as glufosinate, the active ingredient of herbicides such as Basta and Liberty.
EN
Additionally the bar-gene from Streptomyces hygroscopicus is present as a selectable marker gene. The gene encodes phosphinotricin acetyl transferase enzyme (PAT), which acetylates phosphinotricin, also known as glufosinate, the active ingredient of herbicides such as Basta and Liberty.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
Zea mays breeding line: inbred line B104
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pMBb7Fm21GW-UBIL
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- Agrobacterium-mediated DNA transfer
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0.225 kb
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1.134 kb
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1.985 kb
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0.267 kb
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0.551 kb
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0.090 kb
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0.942 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-111070-1 Gibberellin 2-beta-dioxygenase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Changes in physiology and/or production (Growth rate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
Two gene cassettes are present in the modified maize:
I. GA20oxidase
Transcription of the Arabidopsis thaliana gibberellin 2-beta-dioxygenase (GA20 oxidase) is under control of the Zea mays ubiquitin gene promoter and the Cauliflower Mosaic Virus (CaMV) 35S terminator.
II. bar
Transcription of the herbicide tolerance gene phosphinothricin N-acetyltransferase (bar; pat) from Streptomyces hygroscopus commences from the CaMV 35S promoter and ends at the Agrobacterium tumefaciens nopaline synthase terminator. The transcript contains a Tobacco Mosaic Virus 5' untranslated omega leader sequence that promotes the translation of PAT.
EN
I. GA20oxidase
Transcription of the Arabidopsis thaliana gibberellin 2-beta-dioxygenase (GA20 oxidase) is under control of the Zea mays ubiquitin gene promoter and the Cauliflower Mosaic Virus (CaMV) 35S terminator.
II. bar
Transcription of the herbicide tolerance gene phosphinothricin N-acetyltransferase (bar; pat) from Streptomyces hygroscopus commences from the CaMV 35S promoter and ends at the Agrobacterium tumefaciens nopaline synthase terminator. The transcript contains a Tobacco Mosaic Virus 5' untranslated omega leader sequence that promotes the translation of PAT.
EN
- Food
- Feed
- Biofuel
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 2 | |
| Living Modified Organism | Recipient Organism or Parental Organisms | 1 | |
| Living Modified Organism | Related LMO(s) | 1 | |
